Mitigating scoring errors in microsatellite data from wild populations
نویسندگان
چکیده
منابع مشابه
Mitigating scoring errors in microsatellite data from wild populations
Microsatellite data are widely used to test ecological and evolutionary hypotheses in wild populations. In this paper, we consider three typical sources of scoring errors capable of biasing biological conclusions: stuttering, large-allele dropout and null alleles. We describe methods to detect errors and propose conventions to mitigate scoring errors and report error rates in studies of wild po...
متن کاملIdentification of microsatellite markers associated with agronomic and morphological traits in wild einkorn wheat (Triticum boeoticum L.) populations
This study was carried out to investigate the relationship between microsatellite markers and some agronomic and morphological traits in wild einkorn wheat populations collected from western parts of Iran. Plant materials included 163 wild einkorn wheat accessions selected from 664 samples collected from 34 regions in of Ilam, Kermanshah and Kurdistan provinces. Seeds of selected accessions wer...
متن کامل: software for identifying and correcting genotyping errors in microsatellite data
DNA degradation, low DNA concentrations and primer-site mutations may result in the incorrect assignment of microsatellite genotypes, potentially biasing population genetic analyses. MICRO CHECKER is WINDOWS ®-based software that tests the genotyping of microsatellites from diploid populations. The program aids identification of genotyping errors due to nonamplified alleles (null alleles), shor...
متن کاملMicrosatellite variation within and among populations of Oryza officinalis (Poaceae), an endangered wild rice from China.
Oryza officinalis Wall. ex Watt. is an agriculturally important but seriously endangered species of wild rice. To obtain more accurate estimates of population structure for improved conservation planning of the species, genetic variability at 14 microsatellite DNA loci was examined in population samples covering most of the species' range in China. Considerable genetic variability (overall Na =...
متن کاملParentage Tests in Khuzestan Buffaloes Populations Using Three Microsatellite Markers
Pedigree information and phenotypic records are the key features in animal genetics and breeding. Otherwise best methods for genetic evaluation and estimation of parameters will be tending to no correct results. This study was aimed to investigate and verify pedigree relations among 76 individuals who were genotyped for 3 fluorescent labeled microsatellite markers picked up from FAO-ISAG joint ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Ecology Notes
سال: 2006
ISSN: 1471-8278,1471-8286
DOI: 10.1111/j.1471-8286.2006.01449.x